{"created":"2023-06-20T14:15:12.075831+00:00","id":1149,"links":{},"metadata":{"_buckets":{"deposit":"2b6fb917-bf99-4876-8b1b-97d70cc88dbd"},"_deposit":{"created_by":3,"id":"1149","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1149"},"status":"published"},"_oai":{"id":"oai:niu.repo.nii.ac.jp:00001149","sets":["3:4:46"]},"author_link":["3722","3723"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"252","bibliographicPageStart":"247","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"長崎国際大学論叢"},{"bibliographic_title":"Nagasaki International University Review","bibliographic_titleLang":"en"}]}]},"item_10_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHOYAMA, Yukihiro"}],"nameIdentifiers":[{"nameIdentifier":"3723","nameIdentifierScheme":"WEKO"}]}]},"item_10_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_24":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"It has been well known that Aristolochiaceae species induce an endemic nephrosis that a toxic principle is aristolochic acid. In order to set up a sensitive assay system a monoclonal antibody (MAb) against aristolochic acid has been prepared. Anti-aristolochic acid MAb can open the eastern blotting for aristolochic acid resulted that only aristolochic acid appeared on a polyether-sulphon-membrane. We succeeded immunohistochemical analysis of aristolochic acid in mice kidney tissues after injection of aristolochic acid using anti-aristolochic acid MAb. The immunoprecipitation technique using anti-aristolochic acid MAb has been applied to isolate, purify and concentrate a target protein, α-actinin-4 from human kidney cell line in conjunction with mass spectrometry.","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"古来よりｳﾏﾉｽｽﾞｸｻ科植物による腎炎が知られておりその原因物質はｱﾘｽﾄﾛｷｱ酸で有ることが明らかになってきた｡ｱﾘｽﾄﾛｷｱ酸に対する高感度な分析法を開発するために､ﾓﾉｸﾛｰﾅﾙ抗体の作成を行った｡それを用いてｱﾘｽﾄﾛｷｱ酸に対するｲｰｽﾀﾝﾌﾞﾛｯﾃｲﾝｸﾞ法を開発した｡本法では多くの含有成分の中からｱﾘｽﾄﾛｷｱ酸のみが検出出来る｡ﾏｳｽにｱﾘｽﾄﾛｷｱ酸を投与して腎組織をﾓﾉｸﾛｰﾅﾙ抗体で染色するとｱﾘｽﾄﾛｷｱ酸の分布を確認出来た｡さらにﾋﾄ腎細胞にｱﾘｽﾄﾛｷｱ酸を添加して培養し､抗ｱﾘｽﾄﾛｷｱ酸ﾓﾉｸﾛｰﾅﾙ抗体と免疫沈澱法によりﾀｰｹﾞｯﾄﾀﾝﾊﾟｸを精製し､加水分解後ﾏｽｽﾍﾟｸﾄﾙによりα-ｱｸﾁﾆﾝ-4 と同定した｡"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎国際大学"}]},"item_10_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11766493"}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-4094"}]},"item_10_subject_25":{"attribute_name":"Keyword","attribute_value_mlt":[{"subitem_subject":"Aristolochiaceae, aristolochic acid, nephrosis, monoclonal antibody, eastern blotting, targeting protein, α-actinin-4","subitem_subject_scheme":"Other"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"正山, 征洋"},{"creatorName":"ｼｮｳﾔﾏ, ﾕｷﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-14"}],"displaytype":"detail","filename":"RN17-023.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"RN17-023","url":"https://niu.repo.nii.ac.jp/record/1149/files/RN17-023.pdf"},"version_id":"c25824a7-9b15-40e1-8bc2-ac5d5e6e0ed4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ｳﾏﾉｽｽﾞｸｻ科"},{"subitem_subject":"ｱﾘｽﾄﾛｷｱ酸"},{"subitem_subject":"腎炎"},{"subitem_subject":"ﾓﾉｸﾛｰﾅﾙ抗体"},{"subitem_subject":"ｲｰｽﾀﾝﾌﾞﾛｯﾃｲﾝｸﾞ"},{"subitem_subject":"ﾀｰｹﾞｯﾄﾀﾝﾊﾟｸ"},{"subitem_subject":"α-ｱｸﾁﾆﾝ-4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"薬害をひきおこした薬草","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"薬害をひきおこした薬草"},{"subitem_title":"Herbal medicine inducing harmful effects","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["46"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-01"},"publish_date":"2017-04-01","publish_status":"0","recid":"1149","relation_version_is_last":true,"title":["薬害をひきおこした薬草"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-21T06:13:09.916710+00:00"}