{"created":"2023-06-20T14:15:02.280664+00:00","id":770,"links":{},"metadata":{"_buckets":{"deposit":"c12facbe-304d-426a-9ae9-6afa5d62761d"},"_deposit":{"created_by":3,"id":"770","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"770"},"status":"published"},"_oai":{"id":"oai:niu.repo.nii.ac.jp:00000770","sets":["3:4:19"]},"author_link":["1993","1994"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"274","bibliographicPageStart":"265","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"長崎国際大学論叢"},{"bibliographic_title":"Nagasaki International University Review","bibliographic_titleLang":"en"}]}]},"item_10_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHOYAMA, Yukihiro"}],"nameIdentifiers":[{"nameIdentifier":"1994","nameIdentifierScheme":"WEKO"}]}]},"item_10_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_24":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Marihuana is produced from Cannabis sativa L.(Cannabinaceae) and called variously as haschisch, bengh, antabamik, muraba, and so on in the world. Marihuana contains typical alkyl phenolics named as cannabinoid. Among them tetrahydrocannabinol(THC)is the most strong hallucinativelly active cannabinoid. Biosynthetic enzymes of cannabinoids like tetrahydrocan-nabinolic acid synthase(THCAsynthase) has been purified and isolated from the fresh Cannabis plant and its characterization has become evident. THCAsynthase can catalyze the formation of cyclic ring of THCA from cannabigerolic acid(CBGA). Since THCAsynthase no need any co-factor like FAD, NADP NADP and FMN, it seems to be that THCAsynthase itself has co-factor in a molecule. THCAsynthase gene was cloned and super expressed using insect cell system resulting in crystallization. X-ray analysis of THCAsynthase has been succeeded to make evident its whole structure. It became evident that THCAsynthase posses FAD conjugated with histidine and systeine as a co-factor and tyrosine residue in a pocket functions as an acceptor of hydrogen from a hydrogen oxide in CBGA. Granular hair of Cannabis plant accumulated THCA because THCAsynthase was biosynthesized and expressed in only granular hair.","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マリファナはアサから生産され、世界各国地域により色々な名称で呼ばれる。マリファナは特異のカンナビノイドと称するアルキールフェノールを含有する。それらの中で最も強い幻覚活性を持つのが THC である。THCA 生合成酵素を新鮮なアサから精製・単離し、その性状を明らかにした。THCA 生合成酵素は CBGA から環を形成して THCA を生成する過程を触媒する。また、THCA 生合成酵素はいかなる補酵素も要求しないので内在性の FAD 等の補酵素を持つことが予想される。そこで THCA 生合成酵素をクローニングし、昆虫細胞系で大量発現し結晶化に成功した。X-線結晶解析により全構造を明らかにした。これにより FAD がヒスチジン、システインと結合しポケット付近に位置すること、チロシンが環形成に必須であることを明らかにした。THCA 生合成酵素はアサの腺毛で生合成されそこで大麻成分を生合成することが明らかとなった。"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎国際大学"}]},"item_10_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11766493"}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-4094"}]},"item_10_subject_25":{"attribute_name":"Keyword","attribute_value_mlt":[{"subitem_subject":"Marihuana, cannabinoid, THCAsynthase, X-ray analysis","subitem_subject_scheme":"Other"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"正山, 征洋"},{"creatorName":"ｼｮｳﾔﾏ, ﾕｷﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-16"}],"displaytype":"detail","filename":"RN08-025.pdf","filesize":[{"value":"783.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"RN08-025","url":"https://niu.repo.nii.ac.jp/record/770/files/RN08-025.pdf"},"version_id":"33293191-f478-47cb-b681-189faab79bab"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"大麻"},{"subitem_subject":"カンナビノイド"},{"subitem_subject":"THCA 生合成酵素"},{"subitem_subject":"Ｘ線構造解析"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"大麻研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大麻研究"},{"subitem_title":"Study of Marihuana","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-01"},"publish_date":"2015-04-01","publish_status":"0","recid":"770","relation_version_is_last":true,"title":["大麻研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-21T06:18:02.154217+00:00"}