{"created":"2023-06-20T14:15:02.837363+00:00","id":779,"links":{},"metadata":{"_buckets":{"deposit":"01ec67c7-2dab-4f17-8208-8d120fa59235"},"_deposit":{"created_by":3,"id":"779","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"779"},"status":"published"},"_oai":{"id":"oai:niu.repo.nii.ac.jp:00000779","sets":["3:4:20"]},"author_link":["2027","2028"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"73","bibliographicPageStart":"61","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"長崎国際大学論叢"},{"bibliographic_title":"Nagasaki International University Review","bibliographic_titleLang":"en"}]}]},"item_10_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHOYAMA, Yukihiro"}],"nameIdentifiers":[{"nameIdentifier":"2028","nameIdentifierScheme":"WEKO"}]}]},"item_10_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_24":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We investigated the improvement effect of crocin for ethanol-induced impairment of learning behaviors of mice in passive avoidance tasks.　Further it became evident that crocin prevents the inhibitory effect of ethanol on long-term potentiation（LTP）in the dentate gyrus in vivo.　We confirmed that crocin inhibits tumor necrosis factor（TNF）-α-induced apoptosis of PC-１２ cells.　PC-１２ cells rapidly increased the cellular ceramide levels, followed by an increase in the phosphorylation of c-jun kinase（JNK）leading to apoptosis by serum/glucose deprivation in medium.　The production of ceramide was dependent on the activation of magnesium-dependent neutral sphingomyelinase（N-SMase）, but not on de novo synthesis.　The oxidative stress also decreased the cellular levels of glutathione（GSH）, which is the potent inhibitor of N-SMase.　Crocin treatment resulted in the prevention of N-SMase activation, ceramide production, and JNK phosphorylation.　Exploration of the crocin's preventive mechanism for oxidative stress-induced cell death revealed that the activities of GSH reductase and γ-glutamylcysteinyl synthase（γ-GCS）in the γ-glutamyl cycle affected to the stable GSH supply that blocks the activation of N-SMase.　These results strongly support the importance of the proposed GSH-dependent inhibitory mechanism in oxidative stress-mediated cell death.","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"サフランとその主活性成分であるクロシンの薬理作用を検討した。サフランエキスは、アルコールにより記憶学習を障害したマウスの記憶学習能を改善した。本活性本体はクロシンであり、クロセチンに結合するグルコースの数が少なくなるにつれて活性が低下した。PC-１２ 細胞を用いてクロシンのアポトーシス抑制効果を検討した。アポトーシス惹起条件下においてもクロシンを培地へ添加することによりアポトーシスが抑制されることが明らかとなった。クロシンのアポトーシス抑制効果について各アポトーシス誘導ステップを検討した結果、クロシンはスフインゴミエリネース活性を低下することによりセラミドレベルを低下させ、また、グルタチオンレベルを上昇させることにより細胞の安定化を促進し、PC-１２ 細胞のアポトーシスを抑制することが明らかとなった。"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎国際大学"}]},"item_10_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11766493"}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-4094"}]},"item_10_subject_25":{"attribute_name":"Keyword","attribute_value_mlt":[{"subitem_subject":"Crocus sativus L, crocin, memory and learning activity, LTP, neuroprotective effect, neuronal apoptosis","subitem_subject_scheme":"Other"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"正山, 征洋"},{"creatorName":"ｼｮｳﾔﾏ, ﾕｷﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-14"}],"displaytype":"detail","filename":"RN09-007.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"RN09-007","url":"https://niu.repo.nii.ac.jp/record/779/files/RN09-007.pdf"},"version_id":"14891e25-48ab-429f-be59-9041623e8fb1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"サフラン"},{"subitem_subject":"Crocus sativus L."},{"subitem_subject":"クロシン"},{"subitem_subject":"記憶学習"},{"subitem_subject":"長期増強"},{"subitem_subject":"アポトーシス"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"サフランの薬理活性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"サフランの薬理活性"},{"subitem_title":"Pharmacological Activities of Saffron","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-01"},"publish_date":"2015-04-01","publish_status":"0","recid":"779","relation_version_is_last":true,"title":["サフランの薬理活性"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-21T06:17:12.274774+00:00"}