{"created":"2023-06-20T14:15:02.901805+00:00","id":780,"links":{},"metadata":{"_buckets":{"deposit":"e5aa4771-22e2-40b1-9686-f8547e2f40ca"},"_deposit":{"created_by":3,"id":"780","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"780"},"status":"published"},"_oai":{"id":"oai:niu.repo.nii.ac.jp:00000780","sets":["3:4:20"]},"author_link":["2029","2032","2034","2030","2033","2031"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"84","bibliographicPageStart":"75","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"長崎国際大学論叢"},{"bibliographic_title":"Nagasaki International University Review","bibliographic_titleLang":"en"}]}]},"item_10_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MORINAGA, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"2032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"UTO, Takuhiro"}],"nameIdentifiers":[{"nameIdentifier":"2033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SHOYAMA, Yukihiro"}],"nameIdentifiers":[{"nameIdentifier":"2034","nameIdentifierScheme":"WEKO"}]}]},"item_10_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_24":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Sennoside A（SA）and sennoside B（SB）are the major purgative constituents of rhubarb and senna. Intestinal bacteria metabolize sennosides into rheinanthrone, which acts directly on the intestines as a purgative, functioning in the same way as natural prodrugs. Therefore, phytochemical studies of such plants require simple assays for the analysis of SA and SB, such as immunoassay systems that use monoclonal antibodies (MAbs) against bioactive compounds of low molecular weight. In fact, these methods are important tools for the qualitative and quantitative analysis of active constituents from natural medicines and plants. The production and characterization of anti-SA and anti-SB MAbs have been performed in our ongoing study of the production of MAbs against naturally occurring bioactive compounds. As an extension of antibodies generation, we report here the immunoassay systems as indicated below. 1. Establishment of an ELISA and immunochromatographic assays for SA and SB. 2. Development of eastern blotting technique for identification of SA and SB. 3. Immunohistochemical localization of SA in fresh rhubarb root.","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"センノサイドＡおよびセンノサイドＢは、大黄やセンナの主有効成分で瀉下作用をもつ。腸内細菌はセンノサイドをレインアンスロンに代謝し、レインアンスロンが下剤効果を発揮する。大黄やセンナの品質を管理するために、センノサイドＡ、Ｂの簡便な分析方法が必要である。モノクローナル抗体を用いたイムノアッセイは迅速、簡便かつ高感度であり、様々な医薬品成分の分析方法として重要である。著者らはこれまでに、天然由来の生理活性成分に対するモノクローナル抗体作製の一環として、センノサイドＡ、Ｂに対するモノクローナル抗体の作製を行ってきた。その応用として、以下に示すセンノサイドＡ、Ｂの免疫化学的測定法の開発を行ったので報告する。　１.　ELISA 法やイムノクロマトグラフ法を用いたセンノサイドＡ、Ｂのアッセイ系の確立　２.　センノサイドＡ、Ｂのイースタンブロッティング法の開発　３.　免疫組織染色による大黄切片中のセンノサイドＡの分布"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎国際大学"}]},"item_10_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11766493"}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-4094"}]},"item_10_subject_25":{"attribute_name":"Keyword","attribute_value_mlt":[{"subitem_subject":"Sennoside A and B, Rhubarb, Senna, Immunochemical method","subitem_subject_scheme":"Other"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森永, 紀"},{"creatorName":"ﾓﾘﾅｶﾞ, ｵｻﾑ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宇都, 拓洋"},{"creatorName":"ｳﾄ, ﾀｸﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"正山, 征洋"},{"creatorName":"ｼｮｳﾔﾏ, ﾕｷﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-14"}],"displaytype":"detail","filename":"RN09-008.pdf","filesize":[{"value":"871.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"RN09-008","url":"https://niu.repo.nii.ac.jp/record/780/files/RN09-008.pdf"},"version_id":"01014f8c-9369-4250-a32a-8215201caeb0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"センノサイドＡ及びＢ"},{"subitem_subject":"大黄"},{"subitem_subject":"センナ"},{"subitem_subject":"免疫化学的測定法"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"センノサイドＡ、Ｂの免疫化学的測定法の開発について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"センノサイドＡ、Ｂの免疫化学的測定法の開発について"},{"subitem_title":"Development of Immunochemical Method for Sennoside A and B","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-01"},"publish_date":"2015-04-01","publish_status":"0","recid":"780","relation_version_is_last":true,"title":["センノサイドＡ、Ｂの免疫化学的測定法の開発について"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-21T06:17:11.926068+00:00"}