{"created":"2023-06-20T14:15:04.292629+00:00","id":804,"links":{},"metadata":{"_buckets":{"deposit":"3a58b420-8282-4a47-a134-219df0b025a2"},"_deposit":{"created_by":3,"id":"804","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"804"},"status":"published"},"_oai":{"id":"oai:niu.repo.nii.ac.jp:00000804","sets":["3:4:21"]},"author_link":["2128","2127"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"237","bibliographicPageStart":"227","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"長崎国際大学論叢"},{"bibliographic_title":"Nagasaki International University Review","bibliographic_titleLang":"en"}]}]},"item_10_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHOYAMA, Yukihiro"}],"nameIdentifiers":[{"nameIdentifier":"2128","nameIdentifierScheme":"WEKO"}]}]},"item_10_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_24":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"In order to control the quality of crude drugs Aconitum species was clonally micropropagated by tip tissue culture. The clonally propagated plants were cultivated to make clear their homogeneity and I found that wide differences of aconitine-type alkaloid occurred depending on seasonal variation. To confirm the relation between cultivation temperature and the concentration of aconitine-type alkaloid the clonally propagated Aconitum species were cultivated in biotron resulted that the higher temperature induced the higher concentration of aconitine-type alkaloids. The flower buds of Panax ginseng (ginseng) were cultured to produce callus which regenerated via somatic embryogenesis. The homogeneity of regenerated ginseng plants was confirmed by RAPD analysis using suitable primers.  Anti-herbicide gene was induced directly into the Pinellia cells by a particle gun to give the transgenic plants which possess the anti-herbicide activities. A gene of single chain fragment-variable (scFV) antibody against solasodine glycosides was cloned and inserted into the host plant, Solanum khasianum using Agrobacterium plasmid. We confirmed the expression of scFV protein in the transgenic S. khasianum plants by the western blotting and MALDI-tof mass spectrum and the concentration of solasodine glycosides by ELISA used anti-solamargine monoclonal antibody (MAb). The concentration of solasodine glycosides increased approximately three times compared to that of original plant. From this result this methodology can be used as a new breeding technique and we named it missile type molecular breeding. ","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"薬用植物の均質化を遂行するためにトリカブトのクローン増殖を行い、それらクローン苗を栽培してアコニチン系アルカロイドの均質性を確認すると共に、季節変動により含量に大きな差が出ることを明らかにした。また、バイオトロンにより温度による変動を検討し、温度が高いほどアコニチン系アルカロイド含量が高いことを明らかにした。ニンジンの花芽をカルス化し、不定胚から幼植物を得る手法を確立した。本手法により得られた植物の遺伝子分析によりその均質性を確認した。　除草剤耐性遺伝子をパーテイクルガンにより直接カラスビシャク細胞へ導入し、トランスジェニック植物に除草剤耐性が付与されることを明らかにした。抗ソラソジン配糖体モノクローナル抗体の小型化抗体(scFV)遺伝子をホスト植物へ、アグロバクテリュウムを介して形質導入し、トランスジェニック植物を得て、scFVタンパク発現の確認とソラソジン配糖体含量を分析した。その結果scFV遺伝子を導入することによりコントロールに比べて約３倍の含量になった。本手法は新しい育種法として展開可能と考えられ、ミサイルタイプ分子育種と命名した。"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎国際大学"}]},"item_10_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11766493"}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-4094"}]},"item_10_subject_25":{"attribute_name":"Keyword","attribute_value_mlt":[{"subitem_subject":"Clonal micropropagation, particle gun, transgenic plant, scFVgene, missile type molecular breeding","subitem_subject_scheme":"Other"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"正山, 征洋"},{"creatorName":"ｼｮｳﾔﾏ, ﾕｷﾋﾛ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-14"}],"displaytype":"detail","filename":"RN10-024.pdf","filesize":[{"value":"652.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"RN10-024","url":"https://niu.repo.nii.ac.jp/record/804/files/RN10-024.pdf"},"version_id":"3b8e9a91-a445-4669-a004-07d95088a853"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クローン増殖"},{"subitem_subject":"パーテイクルガン"},{"subitem_subject":"トランスジェニック植物"},{"subitem_subject":"scFV遺伝子導入"},{"subitem_subject":"ミサイルタイプ分子育種"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"植物バイオテクノロジーによる薬用植物の育種研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物バイオテクノロジーによる薬用植物の育種研究"},{"subitem_title":"Breeding of Medicinal Plants by Plant Biotechnology","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["21"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-01"},"publish_date":"2015-04-01","publish_status":"0","recid":"804","relation_version_is_last":true,"title":["植物バイオテクノロジーによる薬用植物の育種研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-21T06:16:59.539339+00:00"}